Review Article
Hybrid Coffee (Coffea arabica L) Plantlet Production via Indirect Somatic Embryogenesis in Ethiopia: Current Statue and Future Direction
Elyas Gebremariam*
Issue:
Volume 12, Issue 4, December 2024
Pages:
87-94
Received:
13 August 2024
Accepted:
9 September 2024
Published:
18 October 2024
Abstract: Traditional hybrid coffee propagation methods using seed or vegetative cuttings has tremendous limitations. Seed propagation is associated with hand pollination which is time consuming and need large number of skilled labor. On the other hand, vegetative cuttings ensure uniformity, but; cuttings generate relatively low multiplication rates as they can only be obtained from orthotropic branches. Multiplication by tissue culture techniques could provide a best alternative to these traditional methods of coffee propagation as it allowed the production of relatively uniform plantlets on a large scale in a shorter period in any climatic condition. Among tissue culture techniques, indirect somatic embryogenesis of hybrid coffee plays substantial role in rapid industrial scale multiplication of high valued varities. Research on somatic embryogenesis of C. arabica hybrid has been conducted since the late 70s with the objectives to substitute the conventional vegetative propagation of selected varieties. In Ethiopia, in vitro propagation of hybrid coffee is recent phenomenon and research on indirect somatic embryogenesis is already well underway by different research groups. Here, we discussed the current status of coffee research on somatic embryogenesis in general and specifically, we provide recommendations for future research for the establishment of mass propagation protocol for F1 hybrids varities in Ethiopia that utilized wild endogenous lines.
Abstract: Traditional hybrid coffee propagation methods using seed or vegetative cuttings has tremendous limitations. Seed propagation is associated with hand pollination which is time consuming and need large number of skilled labor. On the other hand, vegetative cuttings ensure uniformity, but; cuttings generate relatively low multiplication rates as they ...
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Research Article
Estimation of Yield Loss Due to Potato Late Blight in Lukanga Village of Lubero Territory/Democratic Republic of the Congo
Issue:
Volume 12, Issue 4, December 2024
Pages:
104-112
Received:
8 July 2024
Accepted:
20 August 2024
Published:
31 October 2024
Abstract: Late Blight is one of important diseases of potato. This disease is present in the agro-ecological environment of Lubero. As a matter of concern, this study aims at assessing the level of tolerance of three potato varieties to this disease and estimating the loss in yield. For this reason, an experiment was carried out in the experimental field of the Faculty of Agronomic Sciences of the Adventist University of Lukanga in the main season of 2023, with an experimental device with complete random blocks. Three potato varieties (Carolus, Kinigi and Sarpo mira) were used with fungicide treatment and non-treatment to estimate the yield loss due to Late Blight. The data concerned the rate of attack of foliar Late Blight and the parameters of potato yield under natural infection. The results showed that tolerance varies from a variety to another, considering the three varieties and is very significant (p<0.001). The Carolus variety was more susceptible with a value of the area under the disease progression curve (AUDPC = 1030) and the Sarpo mira variety the most resistant (AUDPC = 0.000). For the loss of yield, it also varies depending on varieties and is proportional to the susceptibility to foliar Late Blight. It was between 2 and 70% respectively for Sarpo mira and Carolus. Sarpo mira variety, being the most resistant, must both be used for sustainable agriculture that respects the environment and taken in potato breeding program.
Abstract: Late Blight is one of important diseases of potato. This disease is present in the agro-ecological environment of Lubero. As a matter of concern, this study aims at assessing the level of tolerance of three potato varieties to this disease and estimating the loss in yield. For this reason, an experiment was carried out in the experimental field of ...
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Research Article
Effects of Mutagenic Treatments on Drought Tolerance Indices of Selected Cowpea Mutant Lines from Niger State, Nigeria
Issue:
Volume 12, Issue 4, December 2024
Pages:
113-118
Received:
2 October 2024
Accepted:
21 October 2024
Published:
14 November 2024
DOI:
10.11648/j.plant.20241204.14
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Abstract: Cowpea (Vigna unguiculata (L.) Walp.) is an important legume crop cultivated in many regions of Nigeria. It serves as a source of protein, nutrients and income for communities. However, its production and yield potential are severely affected by drought which has been a prevalent constraint over the years, posing a significant threat to cowpea production and food security hence the need to develop mutant lines that can withstand drought conditions for continuous sustenance of lives and livelihoods. This study aimed to determine the effects of mutagens on drought tolerance indices in selected cowpea mutant lines treated with gamma irradiated and Ethyl Methane Sulphonate (EMS) collected from the gene bank of Plant Biology, Federal University of Technology Minna, Niger State. These mutant lines were subjected to water stress for two weeks and assessed for drought tolerance potential. The study revealed that gamma rays and EMS had significantly effects on drought tolerance indices. The Geometric Mean Productivity (GMP) ranged from 4.11 (V2G4) to 7.11 (V3EG3). Stress Intensity (SI) values ranged from 0.050 (V1EG4) to 0.585 (V2G4). V1EG4 exhibited the lowest SI. The SSI values of the cowpea mutant lines ranged from 0.43 in genotype V1EG4 to 2.69 in genotype V2G4. It was observed that four mutant lines namely; V1EG4 (0.43), V1G4 (0.47), V3EG3 (0.48), VIEG3 (0.58), V3EG1 (0.95) respectively had SSI value less than one (1). The Tolerance Index (TOL) ranged from 8.60 (V3EG3) to 12.93 (V2G4). The TOL values of the mutant lines V3EG3 exhibited the lowest and in contrast; V2G4 had the highest TOL, Stress Tolerance Index (STI) values of the cowpea mutant lines ranged from 0.407 in genotype V2G4 to 1.949 in genotype V3EG3. Mutant lines VIG4 (1.741) V1EG3 (1.893), V1EG4 (1.816) and V1EG3 (1.893) also had higher STI as compared to mutant lines V2EG4 (0.892), V1EG2 (0.714) V3G4 (0.709) and V3G1 (0.548). YI values ranged from 0.32 in genotype V2G4 to 2.24 in genotype V3EG3. The HM values ranged from 3.86 in genotype V2G4 to 7.08 in genotype V3EG. Drought tolerance indices studied confirms that mutagen treatments such as gamma rays and EMS significantly affected the tolerance and productivity of cowpea positively. Mutant lines such as such as V3EG3, V1G4 and V1EG4 exhibited enhanced drought tolerance as evidenced in their high MP, GMP, and STI values, along with low TOL and SSI values suggesting this mutant lines could be used for improving drought tolerance in cowpea breeding initiatives.
Abstract: Cowpea (Vigna unguiculata (L.) Walp.) is an important legume crop cultivated in many regions of Nigeria. It serves as a source of protein, nutrients and income for communities. However, its production and yield potential are severely affected by drought which has been a prevalent constraint over the years, posing a significant threat to cowpea prod...
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