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Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)

Published in Plant (Volume 5, Issue 3)
Received: 14 July 2017     Accepted: 21 July 2017     Published: 22 August 2017
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Abstract

The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.

Published in Plant (Volume 5, Issue 3)
DOI 10.11648/j.plant.20170503.12
Page(s) 51-60
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Taro Mildew, Phytophtora colocasiae, Biological Control, Antagonist Microorganisms

References
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Cite This Article
  • APA Style

    Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, et al. (2017). Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant, 5(3), 51-60. https://doi.org/10.11648/j.plant.20170503.12

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    ACS Style

    Asseng Charles Carnot; Ebongo Lobe Emmanuel; Nanda Djomou Giresse Ledoux; Akono Ntonga Patrick; Mbida Jean Arthur, et al. Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017, 5(3), 51-60. doi: 10.11648/j.plant.20170503.12

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    AMA Style

    Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, et al. Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott). Plant. 2017;5(3):51-60. doi: 10.11648/j.plant.20170503.12

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  • @article{10.11648/j.plant.20170503.12,
      author = {Asseng Charles Carnot and Ebongo Lobe Emmanuel and Nanda Djomou Giresse Ledoux and Akono Ntonga Patrick and Mbida Jean Arthur and Ngono Ngane Annie and Ambang Zachée and Monkam Tchamaha Fabrice and Djouokep Léonel Gautier},
      title = {Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)},
      journal = {Plant},
      volume = {5},
      number = {3},
      pages = {51-60},
      doi = {10.11648/j.plant.20170503.12},
      url = {https://doi.org/10.11648/j.plant.20170503.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20170503.12},
      abstract = {The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)
    AU  - Asseng Charles Carnot
    AU  - Ebongo Lobe Emmanuel
    AU  - Nanda Djomou Giresse Ledoux
    AU  - Akono Ntonga Patrick
    AU  - Mbida Jean Arthur
    AU  - Ngono Ngane Annie
    AU  - Ambang Zachée
    AU  - Monkam Tchamaha Fabrice
    AU  - Djouokep Léonel Gautier
    Y1  - 2017/08/22
    PY  - 2017
    N1  - https://doi.org/10.11648/j.plant.20170503.12
    DO  - 10.11648/j.plant.20170503.12
    T2  - Plant
    JF  - Plant
    JO  - Plant
    SP  - 51
    EP  - 60
    PB  - Science Publishing Group
    SN  - 2331-0677
    UR  - https://doi.org/10.11648/j.plant.20170503.12
    AB  - The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.
    VL  - 5
    IS  - 3
    ER  - 

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Author Information
  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Phytopathology and Microbiology, University of Yaounde, Yaounde, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

  • Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon

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